Innovative Methodology Three-Dimensional Mapping of Unitary Synaptic Connections by Two-Photon Macro Photolysis of Caged Glutamate
نویسندگان
چکیده
Masanori Matsuzaki, Graham C. R. Ellis-Davies, and Haruo Kasai Division of Biophysics, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, and Center for NanoBio Integration, The University of Tokyo, Tokyo; Precursory Research Organization for Embryonic Science and Technology, Japan Science and Technology Agency, Saitama, Japan; and Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, Pennsylvania
منابع مشابه
Three-dimensional mapping of unitary synaptic connections by two-photon macro photolysis of caged glutamate.
To understand the precise microarchitecture of the cortical circuitry, it is crucial to know the distribution of synaptic connections and their synaptic strengths at the level of a single cell, rather than a group of cells. Here, we describe a new application of two-photon photolysis of caged glutamate that enabled us to induce an action potential in only a small number (about five) of pyramida...
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tons should produce glutamate in proportion to the Functional mapping of neurotransmitter receptors resquare of the light intensity and do so primarily at the quires rapid and localized application of transmitter. focal plane. We tested this strategy in hippocampal pyThe usefulness of caged glutamate for this purpose ramidal neurons by comparing the performance of a has been limited, because ph...
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